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cd14 positive fraction  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 positive fraction
    Cd14 Positive Fraction, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+positive+fraction/CD14-APC/us12227594-1654-10-26
    Average 97 stars, based on 11 article reviews
    cd14 positive fraction - by Bioz Stars, 2026-09
    97/100 stars

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    other:

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: After 48 hours, cells were transferred into a 96-well round-bottom plate, washed once with PBS and incubated with 50 μl of 3 μg/mL of Fc receptor blocking Mouse IgG Isotype Control (ThermoFisher Scientific, Cat. No. 10400C) in PBS.

    Article Title: Bispecific 2+1 contorsbodies
    Article Snippet: Streptavidin Dynabeads® (ThermoFisher Scientific, Cat. No.: 11205D) were coated with biotinylated human FAP (produced in-house, binding capacity of 6.5×104 beads: 0.01 μg of protein) according to the manufacturer's protocol and added to the B cells in a bead to cell ratio of 2:1 in 50 μl of R10 medium.

    Isolation:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Gradient Centrifugation:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Magnetic Beads:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Activation Assay:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Immunoprecipitation:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Western Blot:

    Article Title: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3
    Article Snippet: .. DOI: 10.7554/eLife.10766 22 of 28 dfdy[0][1] = 2*(-2*Ka1*Conc + Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); JacobN dissociation B = (Rmax-yJacob[0]-2*yJacob[1]); dfdy[0][0] = - Kd1 - Ka2*(B - yJacob[0]); dfdy[0][1] = 2*(Ka2*yJacob[0] + Kd2); dfdy[1][0] = Ka2*(B - yJacob[0]); dfdy[1][1] = -2*(Ka2*yJacob[0] + Kd2); Derive association B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(2*Ka1*Conc - Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Derive dissociation B = (Rmax-yDerive[0]-2*yDerive[1]); dydt[0] = B*(- Ka2*yDerive[0]) - Kd1*yDerive[0] + 2*Kd2*yDerive[1]; dydt[1] = Ka2*B*yDerive[0] - 2*Kd2*yDerive[1]; Syk activation, immunoprecipitation and Western blotting Experiments were performed on the human monocytic cell line, THP-1, and primary human monocytes isolated from leukopacks using the Ficoll-Paque Plus density gradient centrifugation followed by a magnetic cell-sorting using anti-CD14 magnetic beads to collect the CD14 positive fraction with an AutoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). ..



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    Miltenyi Biotec cd14 positive fraction
    Cd14 Positive Fraction, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+positive+fraction/CD14-APC/us12227594-1654-10-26
    Average 97 stars, based on 1 article reviews
    cd14 positive fraction - by Bioz Stars, 2026-09
    97/100 stars
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